Fig. 3

ZCWPW1 expression in mouse and human reproductive system. A Real-time polymerase chain reaction analysis (RT-PCR) revealed the expression of ZCWPW1 in the different mouse tissues. Quantification of the RT-PCR results by a histogram according to the cycle threshold value. B ZCWPW1 immunofluorescence staining for different stages of mouse spermatogenesis using mouse testis sections. (Scale bars, 50 μm). C Immunofluorescence staining for different stages of human spermatogenic cells (Scale bars, 5 μm). D The RNA expression levels of ZCWPW1 at different developmental stages in human testes (TPM = transcripts per million; WPC = weeks postconception)