Fig. 5

Endometrial expression of epithelial receptivity markers. Immunohistochemistry for CD166/ALCAM (a to g) and IGF1-R (h to n). Positive staining was observed in glandular epithelium (brown and arrows) and counterstained with Hematoxylin (blue nuclei). (a and h) Human tonsil tissue was used as the positive control. (b and i) Human endometrium with no primary antibody served as the negative control. (c and j) Ovulatory women. (d and k) untreated anovulatory infertile women. (e and l) CC-treated anovulatory infertile women. (f and m) rFSH-treated women. (g) Optical density analysis of CD166/ALCAM in the glandular epithelium a significant higher expression (*P < 0.05) in CC-treated group compared with ovulatory and untreated infertile women. (n) Optical density analysis for IGF1-R showed a significantly lower expression (*P < 0.05) in glandular epithelium of rFSH-treated women compared with the other groups. GE = glandular epithelium; LE = luminal epithelium; S = stroma; OP = ovulatory women; CC = anovulatory infertile patients treated with clomiphene citrate; rFSH = anovulatory infertile patients treated with the recombinant follicle stimulating hormone; UI = anovulatory untreated patients. (Bar = 100 μm)