Table 6 Assays and instruments used to determine constituents of interest from collected blood specimens
Micronutrient | Amount of serum (μL) | Assay method | Instrument used |
|---|---|---|---|
Iron | |||
Ferritin | 200 | Immunoturbidimetric assay method | Cobas C311 Analyzer, Roche Diagnostics |
Transferrin receptor | 200 | Immunoturbidimetric assay method | Cobas C311 Analyzer, Roche Diagnostics |
Hepcidin | 150 | Enzyme-linked immunosorbent assay (ELISA) | Hepcidin-25, Bachem |
Vitamin A (retinol) | 200 | Quantitative-high performance liquid chromatography photodiode array detection | Agilent HPLC, 1200/1260 Infinity Series with UV/PDA detection |
Vitamin D (25(OH)D) | 400 | Electrochemiluminescence protein binding assay | Diasorin Analyzer, LIAISON |
Inflammation | |||
C-reactive protein | 200 | Immunoturbidimetric assay method | Cobas C311 Analyzer, Roche Diagnostics |
Alpha-1-acid glycoprotein | 200 | Immunoturbidimetric assay method | Cobas C311 Analyzer, Roche Diagnostics |