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Table 2 Summary of procedures

From: Short-course Benznidazole treatment to reduce Trypanosoma cruzi parasitic load in women of reproductive age (BETTY): a non-inferiority randomized controlled trial study protocol

Time

Source

Sample

Tests to perform

Storage requirements

Postpartum period

(Consent/

Assent A)

Maternal venous blood

5 ml EDTA

for plasma

ELISA and IHA

Filter paper 4 °C

Plasma/serum − 20 °C

4–8 weeks postpartum

Home visit to provide consent to be randomized (Consent/Assent B)

Before randomization

Clinical assessment

6 months postpartum

Randomization to BZN 30d/150 mg (intervention arm) or BZN 60d/300 mg (control arm)

Before treatment

Maternal venous blood

5 ml

PCR

10 ml blood-Guanidine 4 °C

Once a week

Monitoring adverse events

During treatment

Maternal finger prick/

venous blood

DBS

BZN concentration

Filter paper −20 °C

End of treatment

Maternal venous blood

5 ml

PCR

10 ml blood-Guanidine 4 °C

10 months after treatment

Maternal venous blood

5 ml

PCR

10 ml blood-Guanidine 4 °C