|
Genes
|
Primer Sequences (5′–3′)
|
Product length (bp)
|
|---|
|
A. Real-time RT-PCR primers
| | |
|
VEGF
|
F: ACCCACCCACATACATAC
|
151
|
|
R: CAGCAGTCAAATACATCCAG
|
|
IGF1
|
F: ATGCTCTTCAGTTCGTGTG
|
148
|
|
R: CAATACATCTCCAGCCTCCT
|
|
IGF2
|
F: CCTCTATCCTTGATACAACAGC
|
121
|
|
R: AATTCGTCTGATTGTCCAGG
|
|
H19
|
F: GTGACAAGCAGGACATGAC
|
121
|
|
R: GAAGTAAAGAAACAGACCCGC
|
|
GAPDH
|
F: CTCATTTCCTGGTATGACAACGA
|
122
|
| |
R: CTTCCTCTTGTGCTCTTGCT
|
|
B. Real-time ChIP PCR primers
| |
|
H19-DMR
|
F: CTACAACCAATTCCGTGCCA
|
127
|
|
R: TAGTGTGAAACCCTTCTCGC
|
|
H19-DMR
|
F: TATCTCAGCCAACACAAGGA
|
127
|
|
R: GCCAGACATTAACATTCCCA
|
- F and R are referred as forward and reverse primers respectively
