Table 1 Primer pairs used in this study
Genes | Primer Sequences (5′–3′) | Product length (bp) |
|---|---|---|
A. Real-time RT-PCR primers | ||
VEGF | F: ACCCACCCACATACATAC | 151 |
R: CAGCAGTCAAATACATCCAG | ||
IGF1 | F: ATGCTCTTCAGTTCGTGTG | 148 |
R: CAATACATCTCCAGCCTCCT | ||
IGF2 | F: CCTCTATCCTTGATACAACAGC | 121 |
R: AATTCGTCTGATTGTCCAGG | ||
H19 | F: GTGACAAGCAGGACATGAC | 121 |
R: GAAGTAAAGAAACAGACCCGC | ||
GAPDH | F: CTCATTTCCTGGTATGACAACGA | 122 |
R: CTTCCTCTTGTGCTCTTGCT | ||
B. Real-time ChIP PCR primers | ||
H19-DMR | F: CTACAACCAATTCCGTGCCA | 127 |
R: TAGTGTGAAACCCTTCTCGC | ||
H19-DMR | F: TATCTCAGCCAACACAAGGA | 127 |
R: GCCAGACATTAACATTCCCA |