Table 1 Procedures with blood samples

Birth

Umbilical cord

15 ml EDTA

Stat-Pak.

-

T. Detect,

ELISA*,

Filter paper 4°C, Plasma -20°C*

PCR*

10 ml Guanidine 4°C*

Umbilical cord

5 ml heparinized

Parasitological examination*

Max 6 hours at 4°C

Before hospital discharge*

Maternal, venous in all (+) subjects

15 ml EDTA

Stat-Pak,

-

T. Detect,

ELISA,

Filter paper 4°C, Plasma -20°C,

PCR

10 ml Guanidine 4°C

Follow-up visit at 4–8 weeks after birth*

Infant, venous, or heel stick

2 ml heparinized, 6 capillary tubes (ideally), or at least one capillary tube

Parasitological examination

Max 6 hours at 4°C

Siblings, finger prick

10 μl

Stat-Pak

-

100 μl

ELISA

Filter paper 4°C

Follow-up visit at 10 months after birth*

Infant, venous or heel stick or digital puncture

3 ml EDTA, or at least one Eppendorf EDTA tube and one filter paper

Stat-Pak,

-

T. Detect,

ELISA

Filter paper 4°C, Plasma -20°C