Fig. 4
From: A novel homozygous mutation in the PADI6 gene causes early embryo arrest
Effects of the variant on PADI6 protein and mRNA expression in vitro. a Western blot of PADI6 protein expression in HEK-293T cells transfected with Flag-tagged wild-type and mutant plasmids. GAPDH was used as the internal control. b RT-qPCR analysis of wild-type and mutant PADI6 mRNA level. The bars showed the mean of three separate measurements, and the error denoted the SEM. c Immunofluorescence of wild-type and mutant PADI6. The experiment was performed with three independent biological replicates yielding similar results. **P < 0.01